文档介绍：
miR-22 通过上皮-间质转化调控头颈鳞癌的
侵袭转移#
谭浩蕾1,2,刘勇1,2,朱刚才1,2,李果1,2,黄美玲1,2,王芸芸1,2,田勇泉1,2,张欣1,2**
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(1. 中南大学湘雅医院耳鼻咽喉头颈外科;
2. 耳鼻咽喉重大疾病研究湖南省重点实验室)
摘要:目的探讨 miR-22 对头颈鳞癌细胞侵袭转移的影响,并对其分子机制进行初步探讨。
方法利用脂质体介导的 hsa-miR-22 mimic 及 inhibitor(含阴性对照)分别转染头颈鳞癌
Tu686 细胞,划痕实验及 Transwell 侵袭实验检测其体外迁移及侵袭能力的改变,Western blot
检测上皮-间质转化分子标志物的改变。结果 hsa-miR-22 mimic 能抑制 Tu686 细胞的体外
迁移及侵袭能力,相反 hsa-miR-22 inhibitor 能促进 Tu686 细胞的体外迁移及侵袭能力。且
miR-22 过表达后能在 Tu686 细胞中抑制上皮-间质转化的分子标志物的改变,即 E-cadherin
的表达上调和 Vimentin 的表达下调。结论本研究阐明了 miR-22 对头颈鳞癌 Tu686 细胞的
侵袭和转移能力具有调控作用,且该调控作用的发生与 miR-22 对 EMT 的抑制作用密切相
关。
关键词:头颈鳞癌;miR-22;迁移和侵袭;EMT
中图分类号:
miR-22 regulate the invasion and metastasis of head and
neck squamous cell carcinoma through the epithelial -
mesenchymal transition
TAN Haolei, LIU Yong, ZHU Gangcai, LI Guo, HUANG Meiling, WANG Yunyun,
TIAN Yongquan, ZHANG Xin
(Department of Otorhinolaryngology Head and Neck Surgery,Xiangya Hospital,Central South
University,Changsha,410008)
Abstract: Objective: To investigate the effect of miRNA-22 on the invation and metastasis of head
and neck squamous cell carcinoma, and explore its molecular mechanism. Methods:
liposome-mediated hsa-miR-22 mimic and inhibitor (including negative control) were applied to
transfect Tu686 cells. Alterations of migration and invasion were assayed by scratches and
transwell
experiments. Finally, western