miRNA与植物体细胞胚发生.doc

文档介绍：
miRNA 与植物体细胞胚发生#
龙健梅,伍小萌,郭文武**
(华中农业大学园艺植物生物学教育部重点实验室,武汉 430070)
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摘要:MicroRNAs (miRNAs) 是一类内源非编码的、长度为 21-24 nt 的单链小分子 RNA,
通过剪切靶基因或抑制蛋白翻译在转录后水平上行使其调控功能。miRNA 影响植物生长发
育整个过程,对合子胚发育有重要的调控作用,然而在离体条件下体细胞胚发生的 miRNA
作用机理研究较少,而体细胞胚发生过程重演合子胚发育的途径。本文借鉴 miRNA 对合子
胚发育的调控功能并结合体细胞胚发生相关 miRNA 高通量测序结果讨论其在体细胞胚发生
中可能的作用,重点从体细胞胚发生相关的植物激素信号转导和转录因子阐述 miRNA 对体
细胞胚发生过程可能产生的影响,并对今后体细胞胚发生相关 miRNA 的研究进行了展望。
关键词:植物;miRNA;体细胞胚发生;合子胚
中图分类号:Q943
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The possible role of miRNA on plant somatic embryogenesis
LONG Jianmei, WU Xiaomeng, GUO Wenwu
(Key Laboratory of Horticultural Plant Biology (Ministry of Eduction),Huazhong Agricultural
University, Wuhan 430070)
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Abstract: MicroRNAs (miRNAs) are a class of endogenous non-coding single strand small RNAs
of 21-24 nucleotides (nt) in length, which guide post-transcriptional gene expression via mRNA
cleavage or protein translation repression. miRNAs have beeen shown to play an important role in
plant development, especially in zygotic embryogenesis. However, little is known about
regulatory mechanism of miRNAs in somatic embryogenesis (SE) in the present study. Since the
process of SE is similar with zygotic embryogenesis, the possible role of miRNA on SE is
discussed in this review, which is got from the insight of roles of miRNA on zygotic
embryogenesis and high-throughput sequencing data of miRNA related SE. P