文档介绍：
茸菖胶囊对大鼠癫痫后 BDNF 蛋白表达的
影响#
杨常泉,马融,王伟**
5
(天津中医药大学第一附属医院,天津市,300193)
摘要:目的研究茸菖胶囊对幼年大鼠癫痫后海马脑源性神经营养因子(BDNF)蛋白表达
的干预作用。方法
建立戊四唑点燃癫痫模型,随机分为中药高、中、低剂量组、西药组、
模型组和空白组,用蛋白免疫印迹杂交法检测各组 BDNF 蛋白的变化。结果
其它各组大
10
鼠海马组织 BDNF 蛋白表达显著高于空白组,差异具有统计学意义(P<)。除中低组
外,其他各组大鼠海马组织 BDNF 蛋白表达与模型组比较差异均有统计学意义(P<)。
与西药组比较,中药高剂量组大鼠海马齿状回 BDNF 蛋白表达无统计学意义(P>)。
结论
中药复方茸菖胶囊抗癫痫及改善癫痫后认知障碍机制可能与抑制幼年大鼠癫痫后海
马 BDNF 蛋白表达有关。
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关键词:中医儿科学;癫痫;海马;茸菖胶囊;脑源性神经营养因子
中图分类号:
Effect of Rong Chang Capsule on expressions of BDNF in
hippocampus of epilepsy rat
Yang Changquan, Ma Rong, Wang Wei
(First teaching hospital of Tianjin University of Traditional Chinese Medicine,Tianjin,300193)
Abstract: [Objective] To explore the effect of Rongchang capsule(RCC) on the protein expression of
brain-derived neurotrophic factor(BDNF) in hippocampus after etrazole(PTZ)induced
epileptic seizure in young rats. [Methods] To establish the PTZ kindling model of epilepsy, the rats
were randomly divided into normalgroup,model group,valproic acid sodium(VPA)group and RCC
low,middle,high dosage group. The expression of BDNF protein were detected by the Western blot
hybridization assay. [Results] The expression of BDNF protein in hippocampus of rats in other
groups were significantly higher than those in the blank group, the difference was statistically
significant (P<). In addition