文档介绍：
小麦成熟胚愈伤组织诱导及分化高效体系
的建立#
赵全志,赵珊,江千涛,魏育明**
5
10
(四川农业大学小麦研究所,成都 611130)
摘要:以 L2,L3,16 四种小麦成熟胚为外植体,探究了不同激素浓度组合下小麦
成熟胚脱分化与再分化情况。结果表明,一定浓度的 2,4-D 与 KT 配比有利于胚性愈伤组织
的形成,,4-D++ 组合可作为最佳的诱导培养基选择;细胞
分裂素的浓度影响愈伤组织再分化的效果, ~ ++
ZT+-BA 比较适宜作为分化培养基的激素添加配比;受基因型影响,供试材料中 L2
的组培效果最差,16 最好,可选择 16 作为建立小麦成熟胚愈伤组织诱导及
分化高效培养体系的候选材料。
关键词:小麦; 成熟胚; 激素; 诱导培养; 分化培养
中图分类号:;
15
The Establishment of Efficient Culture System on Callus
Induction and Differentiation from Wheat Mature Embryos
ZHAO Quanzhi, ZHAO Shan, JIANG Qiantao, WEI Yuming
(Triticeae Research Institute, Sichuan Agricultural University, ChengDu 611130)
20
25
30
35
Abstract: Mature wheat embryos of four wheat varieties L2, L3, L4 16 were cultured in
MS medium with bination of auxin and cytokinin. The results showed that the
combination of 2,4-D+ KT+ NAA is beneficial to the formation of
callus in induction medium. The effect of callus redifferentiation is regulated by different
concentration of cytokinin, and ~ NAA+ KT+ ZT+ 6-BA is
more opportune in all differentiation medium. Because of the difference of genotypes,the mature
embryos of wheat cultivar L2 displayed worse, instead, those of L3 16 are better, L3 and
CN16 can be candidate materials in an efficient system of mature wheat em