文档介绍：
Genome Wide Analysis of the Populus WRKY Transcription
Factor Family Allows the Identification of Poplar
PtrWRKY89 Gene Participating in SA Signaling Pathway.#
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Yuanzhong Jiang1, Jingru Zhu1, Yifeng Ma1, Yanjiao Duan1, Jia Yin2, Shenglong Ye1,
Wanxiang Lu3, Di Fan1, Keming Luo1**
(1. Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of
Education, Chongqing Key Laboratory of Transgenic Plant and Safety Control, Institute of
Resources Botany, School of Life Sciences, Southwest University;
2. Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau
Biology, Chinese Academy of Sciences;
3. College of Horticulture and Landscape Architecture, Southwest University)
Abstract: WRKY proteins are a large family of regulators involved in various developmental and
physiological processes, especially in coping with diverse biotic and abiotic stresses. In this study, a
comprehensive analysis of the Populus genomic sequences showed that the structures of 100 predicted
PtrWRKY genes plete WKRY domain. ic analysis revealed that the members
of this superfamily among poplar, Arabidopsis and other species were divided into three groups with
several subgroups based on the structures of the WRKY conserved domains. Various cis-acting
elements related to stress and defense responses were found in the promoter regions of PtrWRKY
genes by promoter predicted analysis. High-throughput transcriptomic analyses identified that 61 of the
PtrWRKY genes were induced by biotic and abiotic treatments, such as Marssonina brunnea, salicylic
acid (SA), methyl jasmonate (MeJA), wounding, cold and salinity. Among these PtrWRKY genes,
transcripts of 41 selected genes were observed in different tissues, including roots, shoots and leaves.
QRT-PCR analysis further confirmed the induced expression of 19 PtrWRKY genes by one or more
stress treatments. The overexpression of a SA-inducible ge