文档介绍：食品与药品 Food and Drug 2013年第15卷第04期 229收稿日期:2013-02-25基金项目:国家“重大新药创制”科技重大专项“山东省重大新药创制中心建设”(2009ZX09301-013)作者简介:盛华刚,讲师,博士,从事中药新制剂的研究 Tel: 0531-89628590 E-mail: shenghuagang@·论 著·壳聚糖做澄清剂对黄芩水提液的纯化工艺研究盛华刚(山东中医药大学,山东济南 250355)摘要:目的研究壳聚糖做澄清剂对黄芩水提液的纯化效果。方法以纯化前后黄芩水提液中黄芩苷含量、浸膏率为考察指标,并结合HPLC指纹图谱,比较离心法、壳聚糖絮凝沉降法和乙醇沉淀法对黄芩水提液的纯化效果。采用L9(34)正交试验设计,确定壳聚糖絮凝沉降法的最佳纯化工艺。结果壳聚糖絮凝沉降法的纯化效果优于乙醇沉淀法和离心法,最佳纯化工艺为药液质量浓度为1 g/mL、1 %壳聚糖溶液的加入量为药液体积的10 %、药液pH值为4。结论壳聚糖絮凝沉降法可用于黄芩水提液的纯化。关键词:黄芩;壳聚糖;纯化工艺;指纹图谱中图分类号:R943 文献标识码:A 文章编号:1672-979X(2013)04-0229-03Study on the Purification Process of Water Extract of ScutellariaRadix with ChitosanSHENG Hua-gang(Shandong University of Traditional Chinese Medicine, Jinan 250355, China)Abstract:Objective To study the puri?cation process of water extract of Scutellaria Radix with chitosan. MethodsThe purification results of centrifugation, chitosan ulating method and ethanol precipitation method pared using the content of baicalin and dry extract rate as investigation indices, combining with HPLC ?ngerprint. The optimum purification process was screened by orthogonal design. Results The purification effect of chitosan ulating method was better than those of centrifugation and ethanol precipitation. The optimum purification process was as follows: the concentration o f water extract of Scutellaria