文档介绍：富硒酵母中含硒分子的提取和硒的种态分析高愈希二蒲云霞'J彭晓敏',吴刚彳(,北京100049;,包头014040)摘要富硒酵母是常见的补硒营养品,其品质与所含硒的种态有关。为了评价富硒酵母晶质,用超声法破碎酵母的细胞最,用考马斯亮兰法测定提取液屮的蛋白浓度以考察细胞破碎程度,用酶解捉収法提収酵母含硒纟I[分,用电感耦合等离子体质谱(ICP-MS)测定硒含量,通过提収液中的硒含量评估提収效率,用反相离子对高效液相色谱与电感耦合等离子休质谱联用技术(HPLGICP-MS)分析酶解液中的小分子硒。%,其中,SeO32'%,65%的硒以易被人体吸收的硒代蛋氨酸形式存在,其中大部分存在于含硒蛋白分子中。结果表明:超声破碎后用酶解提取法适合于分析富硒酵母中硒的结合形态II能很好地避免捉取液内含硒小分子的转化,所研究富硒酵母中硒主要以易被人体吸收的含硒蛋白形式存在,品质较好。关键词:常硒酵母,种态分析,超声破碎酶解提取,HPLC-ICP-MS中图分类号:,0652ExtractionandSpeciationofSelenium-ContainingSpeciesinSelenium-enrichedYeastGAOYu-xi1,PUYun-xia12,PENGXiao-min1,WUGang2(,InstituteofHighEnergyPhysics,ChineseAcademyofSciences,Beijing100049China;,BaotouMedicalCollege,Baotou014040,China)Abstract:Se---enrichedyeast,theSe-containingspecieswereanalyzedasfollowing:thewallsofSe-enrichedyeastweredisruptedbyanultrasonicassistedprocedure,andthentheSe-containingspecieswereextractedwithanenzymolysisprocedure・ThevalidityofdisruptionwasestimatedbasedontheproteinconcentrationinthedisruptingbuffermeasuredwiththeBradfordmethod・TheextractionefficiencywasevaluatedbasedontheseleniumcontentintheextractmeasuredwithICP--containingspeciesinenzymolysissupernatantwasanalyzedwithreversed-phaseion-pairingH