参芪颗粒中黄芪甲苷含量的测定.doc

文档介绍：参芪颗粒中黄芪甲苷含量的测定摘要:目的:建立高效液相色谱-蒸发光散射检测器(HPLC-ELSD) 测定黄芪甲苷含量的方法。方法:采用蒸发光散射检测器( ELSD )以黄芪甲苷为对照品对参芪颗粒中黄芪甲苷进行 HPLC 分析,色谱柱 Agilent C 18 (250 mm × mm,5 μ m) ,流动相乙腈-水( 32: 68 ),流速 mL/min, 柱温 30℃, ELSD 漂移管温度 105 ℃,载气流速 L/min 。结果:黄芪甲苷在 μ g/mL ~ μ g/mL 范围内线性关系良好,回收率为 %, (RSD= %) 。结论: HPLC-ELSD 法具有良好的精密度和重现性,可用于参芪颗粒中黄芪甲苷含量测定的质量控制。关键词:高效液相色谱;蒸发光散射检测器;参芪颗粒;黄芪甲苷中图分类号: R917 文献标识码: A 文章编号: 1672-1098(2012)02-0067-03 收稿日期: 2012-02-14 基金项目:淮南职业技术学院科学研究资助项目( HKJ11-4 ) 作者简介:陈雷(1983-) ,男,安徽怀远人,助教,硕士,研究方向:临床药理。 Measurement of Astragaloside in Shenqi Granules CHEN Lei 1,2 , HUANG Zheng-gui 1( 1. Department of Pharmacology, Wannan Medical College, Wuhu Anhui 241002, China; 2. Department of Pharmacology, Huainan Vocational Technical College, Huainan Anhui 232001, China) Abstract:The research is to establish the method using the high- performance liquid chromatography-evaporative light scattering detection (HPLC-ELSD) to determine the contents of Astragaloside. ELSD was used to measure the contents of Astragaloside in Shenqi granules on the basis of astragaloside standards for control with HPLC analysis. Other conditions were set as follows: Chromatographic column Agilent C 18 (250 mm × mm, 5μ m), mobile phase of acetonitrile-water (32:68), flow rate in mL/min, column temperature at 30℃, ELSD drift tube temperature at 105 ℃, flow rate of carrier gas in L/min. T