文档介绍：Effects ofMeJA on The Synthesis of Jasmonates and FIavOnOidOnS in Soybean roots Feng Siqiong Specialty: Biochemistry andmolecular biology College: College ofLifeScience Supervisor:Professor BinJinhua Submitted Date:May 27th,20 0 ㈣㈣ 074 摘要类黄酮茉莉酸甲酯(MeJA)作为一种信号分子,调节植物生长发育和诱导植物产生系统抗性。本实验室前期研究表明,MeJA对大豆(Glycine max(L.)Mefr.) 结瘤有促进作用。为了了解外施MeJA是否影响根中一些物质的合成从而影响结瘤,本文通过基因表达分析和化学分析的方法探讨豆科植物根部茉莉酸和类黄酮物质合成情况。在基因表达水平,我们选用大邑黑豆为材料,分别用不同浓度的MeJA(O、 l、10和100“M)喷施植株地上部分,用RT—PCR法分析根中内源茉莉酸合成途径丙二烯氧化物合成酶基因GmAOS和异黄酮合成途径异黄酮合成酶基因 GmlFS的表达情况。结果表明,100州MeJA处理大豆能在一定时间内诱导根部的GmAOS表达;对照组、1州和10 gM处理组根部GmAOS基本不表达; GmIFS在处理前后都有表达。所以大豆外施MeJA,根部GmA∞基因表达呈现浓度和时间效应。色谱大豆结果理能呈逐生结脉根后检隆的苜蓿脉根关键酶基因的表达和MeJA的合成,具有浓度和时间效应,并初步推断MeJA诱导根部内源MeJA和异黄酮的合成从而影响结瘤。关键词:茉莉酸甲酯结瘤异黄酮大豆百脉根 II ABSTRACT SYNTHES lSOF ONS INSOYBEAN Major:Biochemistry and molecularbiology Name:Feng Siqiong Supervisor:Pro佗sSor BinJinhua Methyljasmonate(MeJA)is allimportantplant hormone andinvolved in metabolism ofplant,such勰systemic resistanceresponse inducedby themechanical injury and hasfound that acertainconcentration ofMeJA Canpromote nodulation inGlycinemax(L.)Merr。In order torecognize whether substancessynthesized in roots influencenodulation afterexogenous MeJA treatment, flavonoidomandjasmonates inLegume rootsweredetectedby themethods ofgene expressionganalysis andchemicalanalysis. Expression pattern ofendo-jasmonates biosynthesis allene oxidesynthaseenzyme gene GmAOS and endo-flavonoidons biosynthesis isoflavanonesynthaseenzyme gene GmlFS wereanalyzedby RT-PC&In thisthesis,soybean(Glycine max(L.)Merr.), 鹤materials,were sprayed withMeJA(0,l,1 0 and00灿D onthe aerialportion. The resultswereshowedthat,GmA OSexpressionappeared atacertain timealter 00 山MeJA treatmems,but no GmA OSexpression with0,l or0州MeJA treatment. So GmA OS expression was affectedby Abstract WaS foundthatGmlFS expressed beforeandaftertreanments. Headspace solidphase microextractioncoupled gaSchromatography (HS-SPME—GC