文档介绍：乳糖酶基因的克隆及生物信息学分析【摘要】目的:克隆并分析保加利亚德氏乳杆菌中的乳糖酶基因。方法:利用 PCR 技术从保加利亚德氏乳杆菌中克隆出乳糖酶基因、测序并生物信息学分析。结果: 成功的从保加利亚德氏乳杆菌中克隆出全长为 3 024 bp 的乳糖酶基因,利用生物软件分析,推测乳糖酶基因共编码 1 008 个氨基酸,蛋白分子量为 114 KDa ,等电点为 ,氨基酸序列中共有 9 处潜在的糖基化位点。并将此基因与不同来源的乳糖酶基因进行同源性比较。结论: 成功的克隆出乳糖酶基因,并利用生物分析软件对其进行生物信息学分析。了解该酶的性质特征, 为进一步研究及低成本表达该酶奠定基础。【关键词】乳糖酶基因;克隆;生物信息学分析 Clone and bioinformatics analysis of lactase gene WANG Zheng1, 2, MA Wen li1, ZHENG Wen ling1 ( of Gene Project, South Medical University Guangzhou 510510, China; Laboratory of Molecular Biology, Hainan Medical College Haikou 571101, China ) [ ABSTRACT ] Objective: To clone and analyze lactase gene from Lactobacillus delbrueckii bulgaricus. Methods: Cloned lactase gene from Lactobacillus delbrueckii bulgaricus with PCR, made sequencing and bioinformatics analysis. Results: Cloned lactase gene (3 024 bp) essfully. It was presumed that the lactase gene encode 1 008 amino acids, with protein molecule 114 KDa, isoelectric point , 9 potential glycosylation sites in amino acid sequence. Made parison with other lacteses. Conclusion: The lactase gene is cloned essfully and the bioinformatics analysis is made by biological analysis software to investigate its character. It provides foundation for further study and colonization at low cost. [ KEY WORDS ] Lactase gene; Clone; Bioinformatics analysis 乳及乳制品含有丰富的优质蛋白质、脂肪、碳水化合物以及几乎全部已知的维生素和多种矿物质, 还含有免疫球蛋白等抗病因