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第 30卷第 5期昆明理工大学学报( 理工版) Vol. 30 No 5

2005年 10月 Journal of Kunm ing University of Science and Technology ( Science and Technology) Oct. 2005
纳米银材料抗菌效果研究及其安全性初步评价
钟金栋, 夏雪山, 张若愚, 高毅颖
(昆明理工大学生物与化学工程学院,云南昆明 650224)
摘要: 为了确定纳米银材料的抗菌效果及其使用安全性,进行了纳米银材料对大肠杆菌和金黄
色葡萄球菌杀灭实验效果的观察,并通过小鼠急性毒性实验和家兔皮肤刺激性实验以评价其使
用安全性. 结果表明,在 37℃,大于 5. 7μg /cm2的表面涂布纳米银材料或大于 100 mg/L的纳米银
载体浸泡悬液对大肠杆菌和金黄色葡萄球菌作用 1h抑制率均大于 95% ;纳米级银材料对小鼠
经口灌喂 5 000 mg/kg时,小鼠安全,对兔皮肤刺激性积分为 0,属于实际无毒级. 纳米银材料具有
高效抗菌活性并且使用安全.
关键词: 银;纳米;抗菌材料;生物安全性
中图分类号: Q93 - 334 文献标识码: A 文章编号: 1007 - 855X (2005) 05 - 0091 - 03
Study on An tibacter ia l Eff icacy of S ilver Nanoparticles and
Its B iolog ica l Safety Eva lua tion
ZHON G J in do ng, XIA Xue shan, ZHAN G Ruo yu, GAO Yu ying
( Faculty of B ilogical and Chem ical Engineering, Kunm ing University of Science and Technology, Kunm ing 650224, China)
Abstract: To determ ine the anti - bacterial efficacy of silver nanoparticles, the inhibition effect of the silver nan
opaticles on E. coli and us aureaus are researched. The tests of oral toxicity on m ice and skin stimu
lation on rabbit are conducted to evaluate the safety of silver nanoparticles. The results show thatmore than 95%
bacteria are inactivated when Escherichia coli and us aureaus are incubated at 37℃ for one hour in
the culture dish coated with 5. 7μg/cm2 silver nanopaticles ma