文档介绍：
银纳米簇水凝胶的合成及其抗菌性能研究
马芸,沈晓芳,孙秀兰**
(江南大学食品学院, 江苏无锡 214122)
5
10
15
20
25
摘要:以硝酸银和 L-半胱氨酸为反应物,采用紫外光还原法制备了银纳米簇水凝胶,并用
紫外-可见光谱(UV-vis)、圆二色光谱(CD)、傅里叶变换红外光谱(FT-IR)、透射电镜(TEM)、
基质辅助激光解析电离飞行时间质谱(MALDI-TOF)、X 射线光电子能谱(XPS)对其进行了结
构和形貌的表征。同时选择大肠杆菌()和金黄色葡萄球菌()为受试菌株,
研究了本实验所制备的银纳米簇水凝胶的抗菌性能。结果表明:将摩尔比为 :1 的硝酸银
与 L-半胱氨酸混合液经紫外光照射 h,经过 2 h 水凝胶化过程,得到了平均粒径约为
2nm 的银纳米簇水凝胶,且该水凝胶对两种受试菌株都表现出良好的抗菌作用。
关键词:银纳米簇水凝胶;紫外光还原法;抗菌
中图分类号:
Synthesis of silver nanoclusters hydrogel and its
antibacterial property
MA Yun, SHEN Xiaofang, SUN Xiulan
(School of Food Science and Technology,Jiangnan University, JiangSu WuXi 214122)
Abstract: This paper reports a simple and green method for the synthesis of Cysteine capped
silver nanoclusters hydrogel(Ag NCs) by employing UV photoreduction with AgNO3 and
cysteine as precursors. The structure and morphology of the as-prepared Ag NCs hydrogel was
investigated by ultraviolet–visible spectroscopy (UV-vis), circular dichroism (CD),
Fourier-transform infrared (FT-IR), transmission electron microscopy (TEM), MALDI-TOF and
X-ray photoelectron spectroscopy(XPS). The antibacterial activity of the Ag NCs hydrogel against
Escherichia coli(E. coli) and us aureus(S. aureus) was also conducted. The results
demonstrated that Ag NCs hydrogel of 2 nm was synthesized when the mole ratio of silver
nitrate and L-cysteine is :1 and the UV reduction time is hours. Then hydrogelation
occurred at room temperature for 2 hours. The Ag NCs hydrogel thus obtained showed highly
potent antibacterial activity toward both Gram-positive and Gram-negative bacteria.
30
Key words:
silver nanoclusters hydrogel; UV photoreduction; antibacterial property
0 引言
水凝胶是一种三维立体、以化学键或物理交联作用形成的亲水性聚合物网络,它既有液
体的运动性和流动性,又具有固体的形稳性[1]。与共价交联形成的传统水凝胶即化学水凝胶
35
40
相比,物理水凝胶可以通过分子间的非共价相互作用(如π-π堆积作用、疏水作用、氢键作
用以及静电作用)在水溶液中自聚集形成,因此制备过程相对简单温和[2-3]。近年来,将功能
纳米粒子引入到凝胶中,制成纳米凝胶复合材料,已成为人们关注的热点。功能纳米粒子的
引入不仅可以改善水凝胶的物理机械性能,还可以赋予凝胶新的功能,使其在电学、光学、
催化、医药、和生