文档介绍：分类号 S828 学校代码 10129
U D C 学号 08201051
羊鼻蝇蛆病病理学及鼻黏膜免疫学研究
Research of Pathological and Immunology
of Nasal mucosa on Oestrus ovis
申请人:杜长春
学科门类:农学
学科专业:基础兽医学
研究方向:动物传染病与免疫病理学
指导教师:王凤龙教授
论文提交日期:二〇一一年四月
摘要
通过对山西黄土高原区域羊鼻蝇蛆感染情况调查、病理学及鼻黏膜免疫学的研
究,以明确该地区羊鼻蝇蛆的感染率、该病的病理学及鼻黏膜免疫学变化。从春季至
秋季对羊鼻蝇蛆感染情况进行调查,对 307 例绵羊进行羊头剖检,检查羊鼻蝇蛆病的
各龄幼虫感染情况,同时观察病理变化,并采取感染羊的鼻黏膜、咽后淋巴结、脑和
肺组织进行病理组织学检查,采用 ELISA 检测鼻腔分泌物 IgA、IgG 含量,通过流式
细胞仪对鼻黏膜、咽后淋巴结中 T 淋巴细胞亚群进行检测。春季检查 157 例,鼻蝇蛆
感染患羊 34 例,感染率达 22%;秋季检查 150 例,感染患羊 70 例,感染率达 47%;
春秋两季平均感染率为 34%。鼻蝇蛆感染绵羊鼻黏膜出现不同损伤和增生性炎,咽后
淋巴结炎性细胞浸润,有的病例出现肺气肿,脑组织水肿。检出虫体的羊与未检出虫
体羊比较,咽后淋巴结 IgA 分泌细胞和 IgG 分泌细胞呈增高趋势,尤其是淋巴结髓质
中 IgA 分泌细胞和 IgG 分泌细胞明显增多;感染绵羊鼻腔分泌物中 IgA、IgG 水平显
著高于未感染绵羊,咽后淋巴结、鼻黏膜 CD4+水平显著性高于未感染绵羊,鼻黏膜
CD8+水平较高于未感染绵羊,而咽后淋巴结中 CD8+水平二者无明显差异,咽后淋巴结、
鼻黏膜 CD4+/CD8+的比率显著高于未感染绵羊。
关键词:羊鼻蝇蛆;流行病学;病理学;鼻黏膜免疫学
Research of Pathological and Immunology of Nasal mucosa on
Oestrus ovis
Abstract
In order to make clear the area of sheep nasal flies maggots infection,the pathological
changes and nasal mucosa immunology of the disease,we carried out an infection
survey,pathology and nasal mucosa immunology study of Oestrus ovis in the loess plateau
area in Shanxi province from spring to autumn. 307 samples were dissected to check the
infection status of nasal flies maggots larvae, while histopathology methods were used to
observe pathological changes. And then,we examined the sheep nasal mucosa、
retropharyngeal lymph nodes、brain and lung histologic. Then, the content of IgA and IgG
in nasal chambers secretion were tested By ELISA, the T lymphocyte subsets were
examined by using the flow cytomytor. 34 cases were detected in 157 sampled cases in
spring,detection rate was 22%; 70 in 150 were detected in autumn detection rate was 47%;
the average infection rate was 34%. nasal mucosa appeared different injury and
proliferative inflammation, retropharyngeal lymphadenitis cells were i