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http : www. hxtb. org 化学通报 2005 年第 6 期· 104 ·
进展评述
差异蛋白质组学研究技术新进展
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2 2 2 2 孙薇贺福初
(军事医学科学院放射医学研究所北京 100850)
摘要随着多项基因组计划的完成,人们越来越倾向于借助蛋白质组学手段在整体水平研究复
杂蛋白质样品。差异蛋白质组学比较不同生物体在不同时刻状态下蛋白质表达的变化,已广泛应用于
各种研究。相关技术的建立成为蛋白质组学的一个核心技术问题。不同方法对蛋白质差异分析的能力
和原理不同,可分为提供相对含量信息的定量差异分析技术和提供有无信息的定性差异分析技术。此
外,还有专门技术用于蛋白质修饰的差异分析。多种技术手段的结合应用以及自动化、高通量分析已成
为趋势,修饰研究成为新的研究热点,这些均将促进相关技术的进一步发展。本文介绍了差异蛋白组学
研究技术及其进展。
关键词蛋白质组学差异技术
Advances in Differential Proteomics Techniques
Sun Wei , He Fuchu
(Laboratory of Systems Biology , Beijing Institute of Radiation Medicine , Beijing 100850)
Abstract Recent interest in proteomics has been fueled by pletion of multiple genome projects and
ignited by mon need of biologists to rapidly prehensively plex samples of proteins on a
global level. Differential proteomics , focusing on the changes of protein expressions of different cellstissues under
different conditions , has been applied to many research fields. The establishment of differential proteomics techniques
stands on the central stage in proteomics analysis. Every differential proteomics technique has its own principle and
ability for differential analysis , such as quantitative differential proteomics techniques which make relative quantitation
available and qualitative differential proteomics techniques which can only give all none informat