文档介绍：Progress in Nuclear ic Resonance Spectroscopy 38 (2001) 83±114
ate/pnmrs
NMR studies of protein±DNA interactions
N. Jamina,*, F. Tomab
aCEA/INSTN, 91191 Gif sur Yvette Cedex, France
bDeÂpartement de Biologie, Universite d'Evry, bld F. Mitterand, 91025 Evry Cedex, France
Received 1 June 2000
Contents
1. Introduction .................................................................. 84
2. Overview of techniques .......................................................... 84
. Labeling of DNA .......................................................... 85
. Chemical shift changes ...................................................... 86
. Hydrogen exchange rates ..................................................... 88
. Isotope editing and isotope ®ltering ............................................. 88
. Deuteration ............................................................... 89
. Transverse relaxation-optimized spectroscopy (TROSY) .............................. 89
. Long-range distance constraints ................................................ 90
. Dynamics ................................................................ 91
. Hydration ................................................................ 92
3. Selected applications ............................................................ 92
. The helix-turn-helix motif .................................................... 92
. Homeodomain ....................................................... 92
. Lac repressor headpiece ................................................ 99
. Trp repressor ........................................................ 102
. Ets ............................................................... 103
. Myb .............................................................. 104
. Zinc ®ngers .............................................................. 105
. TFIIIA ...................