文档介绍：Hagan Bayley
Engineered protein nanopores in Reviews
biotechnology
Bayley, H.; Cremer, P. S., Stochastic sensors inspired by
biology. Nature 413, 226-230 (2001).
Biochemistry- Bionanotechnology Option
3rd March 2006 Bayley, H.; Jayasinghe, L., Functional engineered channels
and pores. Mol Membrane Biol 21, 209-220 (2004).
Hagan Bayley
Department of Chemistry Astier, Y.; Bayley, H.; Howorka, S., ponents for
University of Oxford nanodevices. Curr Opin Chem Biol 9, 576-84 (2005).
hagan.******@
.uk/bayleygroup/
Some examples of protein nanopores
Working definition of a nanopore:
Pore < 10 nm in diameter
Pore of > 10 nm are quite easy to make by a variety of means and have quite
predictable properties streptolysin O
scalebar 100 nm
~50 monomers per pore
S layer- porous two-dimensional crystal
α-hemolysin- bacterial pore-forming
toxin
1
Hagan Bayley
Emerging applications of engineered
pores:
• molecular sensing (stochastic sensing) • Survey of classes of nanopores
• drug delivery
• therapeutics
• cryoprotection and ation of cells
• components of nanodevices and nanomachines
• tools in basic cell biology
• tools for fundamental chemistry
a) b) c) d)
barrel stave design
Matile and colleagues
• all L amino acids-
e) f) g)
alternate in and out
• length controlled by
synthetic stave
• poor control of
diameter (number of
Examples of various classes of nanopore. a) Helix bundle in the form of a template-assembled synthetic protein (TASP). b) amino acids in arms,
A single subunit of the trimeric porin OmpF (inner diameter ~2 nm). c) The α-hemolysin pore (inner diameter of barrel ~2
nm). d) Nanotube formed from stacked cyclic peptides (inner diameter ~ nm). e) Synthetic barrel based on octiphenyl charge of each stave)
staves with attached peptides (inner diameter ~ nm). f) Electron micrograph of silica nanotubes made in a porous alumina
template (inner diameter . ~