文档介绍：We focus on precise protein quantification
Human Alpha- Fetoprotein (AFP) ELISA Kit
Catalog Number
EA0019
This ELISA kit used for quantitation of human Alpha- Fetoprotein (AFP) concentration
in cell culture supernatant, serum and plasma.
This package insert must be read in its entirety before using this product.
For research use only. Not for use in diagnostic procedures
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We focus on precise protein quantification
Introduction
AFP is a major plasma protein produced by the yolk sac and the fetal liver during fetal
development. It is thought to be the fetal analog of serum albumin. AFP is the most
abundant plasma protein found in the human fetus. Maternal plasma levels peak near
the end of the first trimester, and begin decreasing prenatally at that time, then
decrease rapidly after birth. Normal adult levels are usually achieved by the age of 8
to 12 months. The function of AFP in adult humans is unknown.[1] However, elevated
serum AFP has been found in patients with benign liver diseases (. cirrhosis,
hepatitis) and various cancers (. hepatocellular carcinoma, hepatoblastoma, germ
cell tumors), making it a useful biomarker. AFP measurement has become an
essential screening and monitoring tool for chronic liver diseases and cancers.
Principle of the Assay
This assay employs the quantitative sandwich enzyme immunoassay technique. A
monoclonal antibody specific for human AFP has been pre-coated onto a microplate.
Standards and samples are pipetted into the wells and any AFP present is bound by
the immobi