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HPLC法测定门冬氨酸鸟氨酸的含量.pdf

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文档介绍:Pharmacy Information 药物资讯, 2016, 5(4), 62-66
Published Online November 2016 in Hans.
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Determination of L-Ornithine-L-Asparate by
HPLC
Yu Yu, Haoyu Yuan*, Jianpeng Zeng
416 Hospital of Nucleus Industry Ministry, Chengdu Sichuan


Received: Oct. 20th, 2016; accepted: Nov. 8th, 2016; published: Nov. 15th, 2016

Copyright © 2016 by authors and Hans Publishers Inc.
This work is licensed under the Creative Commons Attribution International License (CC BY).
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Open Access



Abstract
Objective: To establish a HPLC method for assay of L-Ornithine-L-Asparate. Methods: The HPLC
method was conducted on Waters Spherisorb NH2 (250 mm × mm, id 5 μm). The mobile phase
was composed of acetonitrile- mol/L KH2PO4 (60:40) with the flow of mL/min. The detec-
tion wave-length was 210 nm. Results: L-ornithine and L-aspartate showed good linear (r = )
in the range of - mg/mL and - mg/mL, respectively. The average recovery of
L-