文档介绍：2004年第 62卷第 15期, 1379~ 1384 化学学报 ACTA CHIMICA SINICA Vol . 62 , 2004 No. 15 , 1379~ 1384 聚丙烯片基不同气氛下等离子体改性及 DNA原位合成研究汤建新 a,b 周灵君 c 陈洪 a 何农跃Ξ, a,b,d Ξ( a株洲工学院包装新材料与技术重点实验室株洲 412008 ) ( b东南大学教育部分子与生物分子电子学重点实验室南京 210096 ) ( c南京中医药大学药学院南京 210029 ) ( d南京医科大学第一附属医院南京 210029 ) 摘要分别采用氮气/氢气、氨气和氧气三种不同气氛的等离子体处理了聚丙烯片基,先使其表面接枝功能性基团,(XPS ):三种方法处理的聚丙烯片基都可用于 DNA原位合成,但从处理工艺和荧光分析结果来看,以氮气/氢气等离子体处理的聚丙烯片基最佳. 关键词等离子体,聚丙烯,寡核苷酸探针阵列,原位合成 parative Study on insituSynthesis of Oligonucleotide on Different Plasma Modified Polypropylene Surfaces TANG, Jian2 Xin a,b ZHOU , Ling2 Jun c CHEN , Hong d HE, Nong2 Yue Ξ, a,b,d ( aKeyLaboratoryf orPackingMaterialsandNewTechnologyofZhuzhouInstituteofTechnology,Zhuzhou412008 ) ( bKeyLaboratoryf orMolecularandBiomolecularElectronicsofMinistryofEducation,SoutheastUniversity,Nanjing210096 ) ( cCollegeofPharmacy,NanjingUniversityofTraditionalChineseMedicine,Nanjing210029 ) ( dFirstAffiliatedHospital,NanjingMedicalUniversity,Nanjing210029 ) Abstract This pared results of oligonucleotide probe array in2 situsynthesized on the hydrogen and nitrogen plasma modified polypropylene (PP )surface with those on the amino plasma modified PP surface and also with those on oxygen plasma and silanizing modified PP surface. X2 ray photoelectron spectroscopy (XPS )analysis confirmed the presen