文档介绍：第30卷第3期 2009年9月内蒙古农业大学学报 Journal of Inner Mongofia Agrieahural University 5个节旋藻样品基因组DNA含量的测定’邰丽华1, 白洁1,乔辰2 内蒙古师范大学生命科学与技术学院,呼和浩特010022;2. 内蒙古农业大学农学院,呼和浩特010019) 摘要:本文以鄂尔多斯高原碱湖钝顶节旋藻A。、鄂尔多斯节旋藻A:、方胞节旋藻A,、引进的非洲Chad湖钝顶节旋藻A4和墨西哥Texeoco湖极大节旋藻A,为实验材料,采用CTAB一溶菌酶法提取其基因组DNA,紫外分光光度法测定DNA含量。结果表明:/s DW范围内,含量高低排列是A4>A2>AI>As>A3,不同样品DNA含量存在较大的差异。关键词:节旋藻;DNA含量; 紫外分光光度法中国分类号:Q52 文献标识码:A 文章编号:1009—3575(2009)03—0241—03 DETERMINING GENOMIC DNA CONTENT 0F FIVE SPECIES INArthrospira TAI Li—hual,BAI Jiel,QIA0 Chen2 (,Inner Mongolia Normal University,Huhhot 010022,China; ofAgronomy,Inner Mongolia Asriculturd University,Huhhot 010019,China) Abstract: ne genomic DNA offivespecies inArthrospira WaS extracted by CTAB—lysozyme method and contents ofDNA we弛de. termined by me&rl瞎of ultraviolet spectrophotometer,which include Arthrospira platensis(AI),(A2),(A3)from alkaline lakes inErdos Plateau,(A4)from Chad Lake (如)from Texcoco Lake resuhs cate thatthe content of DNA 5species inArthrospira are 184— tendency A4>A2>Al>A5> A,.There aIe obvious differences the content ofDNA between 5 species. Key words: Arthrospira: content of DNA: ultraviolet spectrophotometer 节旋藻(Arthrospira)是1类原核光合生物,为多细胞、不分枝、无异形胞、螺旋状的丝状体,其结构简单、繁殖快、兼具植物和微生物的双重特性,是植物分子生物学、基因工