文档介绍：Isolation and Identification of Two Degrading Strains
in Atrazine Contaminated Soil
Ying Zhang, Chunyan Li, Zhuo Diao, Shuyan Ma, Rongjuan Wang *
School of Resource & Environment, Northeast Agricultural University,
Harbin 150030, China
Email: ******@neau.
Abstract—Two strains named W7 and W16 were isolated by degrading strains isolated from the soil in the north have been
liquid enrichment culture from the corn field where atrazine has inadequate. So searching for the atrazine-degrading strains
been chronically applied. Observed by gram stain and SEM, W7 suitable for bioremediation of the soil in the north are of great
belonged to gram-positive bacteria and the single cell was short significance in enriching the diversity of atrazine-degrading
bacilliform; W16 belonged to gram-negative bacteria and the strains.
single cell was spherical. Through physiological and
identification and 16SrDNA sequence analysis, two strains were This experiment aims at isolating high efficient
identified as Rhizobium sp. and Rhodobacter sphaeroides sp. atrazine-degrading strains from Wuchang cornfield in Harbin
respectively. W7 and W16 could degrade atratine effectively, and City Heilongjiang Province where atrazine has been
the biodegradation rate reaching up to 99 % after 72h chronically applied, and identifying them with 16SrDNA
cultivation in suitable