文档介绍：Molecular Cloning and Bioinformatics Analysis of Duck MRFs
Gene Family Coding Domain Sequences

Liu He-he, Wang Ji-wen, Huang Kai-liang, Si Jian-min, Jia Jing, Li Liang, Xu Feng
(Key Lab of Animal ic Resources, Sichuan Agricultural University, Ya’an 625014, China)

Abstract—Myogenesis in skeletal muscle is a cascade of to know the reasons. These genes had been cloned and
developmental events, whose initiation involves the MRFs family analyzed in the human [5], bovine [6], swine [7], and chicken
of the myogenic regulatory factors. Comparing the [8][9], these data indicated that the secondary structure helix-3
characterization of MRFs family members and their deduced provides a mechanism whereby the MRFs have differentiate
proteins is necessary to know the reasons of their different role in roles in muscle development [10].
muscle development. In order to investigate the reasons why
The Peking duck (Anas platyrhynchos) is famous for its
MRFs members have different roles in duck myogenesis, the
genes MyoD1, Myf5, MyoG and MRF4 in the duck were cloned, fast muscle growth and high production of muscle. However,
and were analyzed using bioinformatics knowledge. The result the duck orthologue of these genes has not yet been found.
shows that these four sequences share a high homology with Therefore, the objectives of this study were to clone the
a