文档介绍：The cloning and analysis of a partial lipase gene
sequence of us hominis
Subtitle: Partial lipase gene sequencing of S. hominis
QIAO Na-na1 (
), GAO Qiang1, 2 (
), ZHAO Qin1, 4, WANG De-pei2, Chen Yi-peng3, ZHAO Wen-yi2,
YU Chang-yan4
1. College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, P. R. China;
2. Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin 300457, P. R. China;
3. The Public health Administration Center of Xiqing District, Tianjin City, Tianjin 300380, P. R. China;
4. Tianjin Key Laboratory of Industrial Microbiology, Tianjin 300457, P. R. China
E-mails: qiaona123@; ******@tust.
Abstract—Lipase activity mon in i. Based on
the blast of various known al lipase DNA sequences, II. MATERIALS AND METHODS
the degenerate primers were derived to amplify a 782-bp
consensus lipase gene sequence of us hominis A. Strains
, which encoded a deduced polypeptide of 260 amino S. hominis was purchased from Guangdong
acid (aa) residues. The alignment of aa sequence revealed that Institute of Microbiology. S. carnosus TM300 was a kindly
this 260-aa partial lipase of S. hominis shared high homology
gift from Prof. Dr. Friedrich Goetz, and S. epidermidis RP62A
with those conserved parts of other 11 deduced al
α
lipases, in the range of the lowe