文档介绍：Effects of high temperature stress on transgene
expression and DNA methylation pattern
Fan-suo Zeng1, Ying Xin2, Kun Liu1, Ya-guang Zhan1 *, Chuan-ping Yang2
1College of Life Science, Northeast Forestry University, Harbin 150040, China
2 College of forestry, Northeast Forestry University, Harbin 150040, China
* Corresponding author: youpractise@
Abstract—Studies about the variation of DNA methylation not only occur during plant exposure to stress, but also
pattern under high temperature stress (37˚C) contribute to a establish numerous changes in gene expression that can persist
comprehensive understanding of the apparent regulation of over several generations [4]. Transgene expression level of
biological functions. Based on the in vitro cultivation of transgenic birch lines in stress was therefore an important
ically modified birch (Betula platyphylla Suk.) clones for consideration for transgenic cultivar development programs.
high-temperature stress, the paper detected exogenous gene The purposes of the study were to: (i) analyse the expression
transcription level differences with RT-PCR. Methylation- level of transgene in transgenic birch in response to stress; (ii)
sensitive amplified polymorphism (MSAP) analysis the variation analyse the effect of high temperature stress on DNA
of DNA methylation pattern in transgenic birch. In this pa