文档介绍:The Research on Proteomic Changes in Two
ulant Producing Bacteria When Grown in
Minimal Media versus ulant Media
Bing Yu1, 2, Fang Ma1, Peishi Qi1, Ang Li1, Lili Wang1
1. State Laboratory of Urban Water Resources and Environment, School of Municipal and Environmental Engineering,
Harbin Institute of Technology, Harbin, China
2. Key Laboratory of Molecular Biology, College of Life Science, Heilongjiang University, Harbin, China
Email: ybgirl1234@, ******@hit.
Abstract—In order to reveal the molecular mechanisms and determined as haride and the output of haride
the metabolic pathway of producing ulant, the changes in in the crude CBF was [4].
plexes of F2 grown in minimal media versus
ulant media and those of F6 grown in minimal media Analysis of changes in bacterial proteomes has been an area
versus ulant media were respectively researched by SDS- of intense research in the last several years. Changes in
PAGE. SDS-PAGE results indicated that the relative band bacterial growth media or conditions can have significant
volumes of three bands were obviously decreased in F2, the effects on protein expression[5].When F2 and F6 were
relative band volume of one band was obviously increased in F2 as respectively grown in minimal media, the output of the
well as the relative band volumes of two bands were obviously ulant was nearly zero