文档介绍：Purification of Nonmitogenic
binant Human Acidic Fibroblast
Growth Factor in Escherichia coli
Hui YUAN Xiao Kun LI
Zhejiang University of Science and Technology Wenzhou Medical College
Key Laboratory of Agricultural Products Bio-natural Pharmaceutiacl School
Chemical and Biological Processing Technology Wenzhou, , 325035
of Zhenjiang Province
Hangzhou, , 310023
dnastar @
I. INTRODUCTION
Abstract—Non-mitogenic form of
binant Human acidic fibroblast growth Acidic fibroblast growth factor (aFGF) was a
factor (nrhaFGF) retained the cardio and powerful and broad-spectrum mitogen, it
neuro-protective characters of the wild-type stimulated all the cells of mesodermal and
[1]
aFGF while decreased the potential side neuroeltodermal origin to divide in vitro , it
effects like mitogenesis activity. In this paper, was also a powerful vasodilator, a very efficient
nrhaFGF was essfully expressed in protector of brain and heart tissues against the
Escherichia coli and yielded about 5% of the effects of ischemic reperfusion, and a potent
[2,3]
total soluble protein in the 10L fermentation neuromodulator , but the mitogenic activity of
tank. The eedent chromatography, cation wild type haFGF has limited its use in clinical
[4]
exchange and heparin affinity gained the greatly . Xiaoping Wu had constructed a system
pur