文档介绍：Preparation of glucan sulfates with different degree of
substitution and their immunoprophylaxis potentials
in Escherichia coli induced mice peritonitis
Liping Du, Hongyan Niu, Xuekuang Zhang, Dongguang Xiao*
Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology
Tianjin University of Science and Technology
Tianjin 300457, China
*Corresponding author. E-mail: ******@tust.
Abstract—Glucan sulfates with different degree of substitution In this paper, soluble glucan sulfates with different degree
were prepared from partially sulphated insoluble β-1,3-D-glucan of substitution (DS) were prepared through partially sulphating
isolated from haromyces cerevisiae cell wall. Sulphation was insoluble β-1,3-D-glucan in the presence of dimethyl sulfoxide
done with sulfate acid (H2SO4) in dimethyl sulfoxide (DMSO) in (DMSO) and urea. Different reaction conditions, such as time,
presence of urea. Varying reaction times, temperature and ratio
temperature and concentration of sulfate acid (H2SO4) were
of H2SO4 and DMSO were used to obtain glucan sulfates with explored. Immunoprophylaxis efficacy of these glucan sulfate
distinct degree of substitution, which was measured by barium derivatives was investigated in a mouse model of Escherichia
chloride-gelatin method. The immunoprophylaxis effect of glucan coli induced