文档介绍：Application of Agilent 2100 Bioanalyzer in Multiplex PCR for
Detection of Foodborne Pathogens*
WANG Bo-fei1, BAI Yan-hong1, ZHAO Pei-pei1, WANG Yun-long2, JING Jian-zhou1*
(1. College of Food Biotechnology Engineer, Zheng Zhou University of Light Industry, Zhengzhou Henan 450002,
China;
2. Henan Bioengineering Technology Research Center, Zhengzhou Henan 450002, China)
E-mail address:wbf_512@
Abstract—To explore the feasibility of using Agilent 2100 Chinese anism fungiform conserve center. Primers of
Bioanalyzer in the detection of the three kinds of food-borne PCR were showed as table 1.
pathogens, three pairs of primers were designed according to [7]
invA gene of Salmonella ipah gene of Shigella and inv gene of Primer sequences :
Yersinia pseudotuberculosis, and then the single PCR was Tab. 1 Result of primer design of PCR
performed to detect specificity. Those genes were amplified by
Multiplex PCR technique using specific primers, and the Target
Pathogens PCR Primer sequences (5'-3')
optimum of multiplex PCR reaction conditions. The multiplex gene
PCR detection method which provided a basis for further invA Sal-3a,ACGTTCGGGCAA
Salmonella spp. 275bp
research on developing the food-borne pathogens detection kit gene Sal-4a,
Shi-1a,GATA
was very practical. Agilent 2100 bined with ipah
Shigella spp. C 610bp
Mul