文档介绍：Preliminary Study on apoptotic effect induced by N-
butanol extract in Capparis spinosa L. on SGC-7901
Lei YU, Le-qiong XIE Yu-bin JI
Institute of Materia Medica and Postdoctoral Programme Engineering Research Center of Natural Anticancer Drugs
Harbin University merce Ministry of Education
Harbin, China Harbin, China
yulei912@ yulei912@
Abstract—The purpose of this article was to investigate the anti-tumor drugs and make full use of Capparis to
apoptosis effect of the n-butanol extract from Capparis spinosa L. play its unique pharmacological activity, this study primarily
(CSBE) on human Gastric Cancer cells SGC-7901. MTT assay explored apoptosis of SGC-7901 induced by CSBE through
was used to measure the inhibitory rate of CSBE Morphological vitro experiment.
changes of apoptosis were observed with fluorescence
microscope. Flow cytometry was employed to analyse the
apoptotic rate of CSBE on SGC-7901 cells. Laser confocal II. METHOD
2+
microscopy(LCM) was adopted to assess [Ca ]i in the cells. The
proliferation of SGC-7901 cells were inhibited after treated with A. Determination of inhibition in SGC-7901 cells through
CSBE in the dose of 5, 25, 50, 100, 200 and 400 μg/mL for 72 h, MTT assay
and the IC50 was μg/mL. After SGC-7901 cells were The MTT assay was performed at first described by
treated by CSBE for 40