文档介绍：MDM2 gene promoter polymorphism and risk of
cervical cancer in Chinese population
Pei Jiang1, 2, Jianxin Liu1, Xiaoxi Zeng2, Wen Li1, Jianxin Tang1, 2
1School of Info-physics and Geomatics Engineering 2Green Packaging and Biological Nanotechnology
Central South University Laboratory
ChangSha 410083, China Hunan University of Technology
Zhuzhou 412008, China
Abstract— Purpose: Mouse double minute 2 (MDM2) is a key [5]. MDM2 has also been shown to promote tumor growth in a
negative regulator of the p53 activity. Recently, a polymorphism TP53-independent manner [6].
in the intronic promoter, SNP309, was shown to influence MDM2 Recently, a functional single nucleotide polymorphism
expression and p53 activity. We examined whether the SNP309 (SNP) in the MDM2 intronic promoter (named SNP309) was
was related to the risk of developing cervical cancer among identified. The cells carrying the SNP309 GG genotype were
Chinese populations. Experimental Design: We genotyped the
MDM2 polymorphism SNP309 of peripheral blood DNA from found to increase the binding affinity of the transcriptional
110 cervical cancer patients and from 160 controls. Genotyping activator Sp1 and subsequently resulted in higher expression
were confirmed by polymerase chain reaction-restriction levels of MDM2 mRNA and pared with those
fragment length polymorphism (PCR-RFLP