文档介绍：脂肪酶固定化办法研究进展
生物工程2班周明 1525
摘要：酶固定化是生物技术中最为活跃研究领域之一。脂肪酶能发生催化水解、醇解、酯化、酯互换等反映，是一种重要生物催化剂。而由于脂肪酶特性，其能否工业化运用很大限度取决于固定化技术成功与否，酶固定化办法是酶固定化技术重要研究内容。固定化脂肪酶由于其易与底物分离且可重复使用而备受关注。为此，本文综述了惯用固定化办法，涉及物理吸附法、共价结合法、交联法和包埋法，不同固定化办法对酶性质有不同影响。本文对近年来固定化脂肪酶办法研究进行了综述，为固定化办法进一步探讨提供了研究基本。
核心词：固定化；脂肪酶；载体材料
The research progress of lipase immobilized
Abstract：Of enzyme fixed is in the biotechnology research field of the most active. Fat enzymes would happen catalytic water and alcohol，ester，ester exchange for is a major catalyst of biological. And the fat，the enzymes can be very much depends on the use of technology on the success of the enzyme is a fixed set of the technical content of important research. Into a fatty because of the enzymes that are and separate and can reuse have a major concern. The common immobilization methods were generally introduced including physical adsorption，covalent cross-linking method and entrapment method. Different immobilization methods had different effects on the enzyme. Of the few years，the enzymes a study of the review，as a further explore the method provides research.
Keywords：immobilization；lipase；carrier
脂肪酶（Lipase ，甘油酯水解酶）是一类特殊酰基水解酶它底物是油脂其水解部位是油脂中脂肪酸和甘油相连接酯键[1]；人们对脂肪酶研究已有上百年历史，是最早被研究酶类之一[2]。脂肪酶作为生物催化剂，可用于许多有机合成反映，列如
能在油—水界面上催化酯水解或醇解、酯合成、酯互换、内酯合成、多肽合成、高聚物合成及立体异构体拆分等有机合成反映，是当前被重点研究酯催化剂[3]。关于脂肪酶(1ipase)较确切定义是20世纪70年代由 Brockerhoff等一方面提出，她将水