文档介绍：中国农业科学 2005,38(11):2227-2232
Scientia Agricultura Sinica
候选抗病基因在水稻品种多样性研究中的应用
孙雁,王云月,何月秋,李成云,周江鸿,朱有勇
(云南农业大学植物病理重点实验室/农业生物多样性国家工程中心/教育部农业生物多样性与病害控制重点实验室, 昆明 650201)

摘要: 利用 19 个来源于水稻和大麦、具有抗病基因 NBS-LRR 结构特点的探针对 24 个水稻品种的 Dra I酶切
DNA 片段进行候选抗病基因分析,用 26 个稻瘟病菌株对供试品种进行人工接种试验。结果表明:供试品种中共检
测到 127 个候选基因位点,平均每个探针为个。除齐头谷、八宝谷和紫糯外,籼稻品种的位点数均高于粳稻
品种,而这些籼稻品种表现较为抗病。聚类分析将供试品种划分为籼粳差异明显的 2 个类群和分群结果与其系谱
关系及抗性基本吻合的 5 个亚群,表明候选抗病基因标记可用于水稻品种的遗传关系、水稻病害控制和抗病育种
方面研究。
关键词:水稻;候选抗病基因;限制性片段长度多态性;多样性;稻瘟病

Application of the Candidate Resistance Genes to
ic Diversity in Rice
SUN Yan , WANG Yun-yue, HE Yue-qiu, LI Cheng-yun, ZHOU Jiang-hong, ZHU You-yong
( China National Center for Agricultural Biodiversity / Key Laboratory of Agricultural Biodiversity for Plant Disease Management,
Ministry of Education / The Center for Agricultural Biodiversity Research & Training of Yunnan Province / Key Laboratory of Plant
Pathology, Yunnan Agricultural University, Kunming 650201)

Abstract: The homologues of 24 rice cultivars were analyzed using 19 candidate resistance gene (CRG) probes from rice and
barley with NBS-LRR structure, while the resistance of the cultivars was tested by inoculating 26 isolates of Magnaporthe grisea in
this study. The results showed that the 24 cultivars had 127 loci totally, each probe had loci averagely. Except for Qitougu,
Babaogu