文档介绍：·626· 2013 7 34 7 Chin J Hematol, July 2013, Vol. 34, No. 7L⁃27 IL⁃27 MM
、 自分泌 ， 可能参与 的发病机制。
27 6;
【关键词】 白细胞介素 ； 白细胞介素 多发性骨髓瘤
Expression of IL- 27 in multiple myeloma and its cell lines XIA Tian*, LI Jian- ping, LI Wen- qian,
WANG Li, HAO Xing, SUN Fang-fang, DAI Xin, A Xiang-ren, PENG Hai, SONG Yu-jun, FENG Jian-ming*.
*Department of Hematology, Qinghai Provincial People's Hospital, Medical College of Qinghai University,
Xining 810001, China
Corresponding author: FENG Jian-ming, Email: ******@
【Abstract】 Objective To study the expression and significance of IL-27 in patients with multiple
myeloma (MM) and in the supernatant of MM cell lines U266 and RPMI8226 cells culture medium.
Methods A total of 20 MM patients and 20 controls were enrolled in this study. The expressions of IL-27
and IL- 6 in MM patient’s blood plasma, and the expression of IL- 27 in U266 and RPMI8226 culture
supernatant were measured by enzyme-linked immunosorbent assay (ELISA). The mRNA expression of IL-
27 in mononuclear cells of MM patients’peripheral blood was measured by real- time quantitative
polymerase chain reaction (RT-PCR). Results The levels of IL-27 in plasma of MM patients and normal
controls