文档介绍：文章编号:100021573(2002)0420134203高效液相色谱法测定辣椒素赵仁邦,崔同,果秀敏,葛微,张冬,刘淑晨(河北农业大学食品系,河北保定 071001)摘要:研究了用高效液相色谱法测定辣椒中的辣椒素。色谱柱为Hypersil BDS C18(5μm, 250 mm×416mm)。UV检测器波长为280 nm。流动相为V(CH3OH)∶V(H2O)= 85∶15 ,流速为016 mLΠmin。结果表明:线性回归方程是Y= 1112X+ 7197,相关系数为r=01999 9,样品的精密度RSD= 216 %,平均回收率是95171。关键词:高效液相色谱;辣椒素;超声波中图分类号: O65717+2文献标识码: ADetermination of capsiciu by high performance liquid chromatographyZHAO Ren2bang,CUI Tong,GUO Xiu2min,GE Wei,ZHANG Dong,LIU Shu2chen(Deqartment of Food , Agricultural University of Hebei , Baoding 071001 , China)Abstract: The Determination of capsiciu which is in pepper was studied by means of high performanceliquid chromatography(HPLC)1The experimental results showed that the capsiciu can be separated fromother pigments by HPLC1The HPLC system was consisted of Hypersil BDS C18(5μm, 250 mm×46mm1)and UV detector set at 280 nm1The mobile phase was V(CH3OH)∶V(H2O)= 85∶15 with aflow rate of 016 mLΠmin1The linear equation wasY= 7197 + 1112Xand correlation coefficients was01999 9. The average recovery was 951711The detection limit was 0167 ng1The experiment showed thatthis method is sensitive , accurate and reliable1