医学检验毕业论文.doc

文档介绍：南通体臣卫校毕业论文题目三种肺炎支原体检测法的临床应用分析地市:南通市学校:体臣卫校准考证号:考生姓名:陆施燕指导老师:2015年5月24日-2-三种肺炎支原体检测法的临床应用分析摘要目的探讨肺炎支原体(MP)咽拭子快速液体培养法、咽拭子聚合酶链反应(PCR)法和血清MP被动凝集法(MP-Ab)等方法在儿童MP感染诊断治疗过程中的敏感性方法采用3MP检测法对362例临床拟诊呼吸道(非细菌性)感染的患儿的咽拭子和血清标本进行配对研究,每患儿取咽拭子做快速培养和PCR,同时取血清做MP-Ab检测,对3种方法的检测结果与临床诊断治疗病例进行回顾性分析。结果回顾临床病例诊断MP感染患儿152例。MP快速培养法检出阳性78例,%,病程为(±)天;PCR法检测出阳性103例,%,病程为(±)天;MP-Ab法检测出阳性127例,%,病程(±)天。结论3种MP检测法的敏感性与病程有相关性,临床医生应根据患儿病程选取检测方法,以提高阳性检出率和敏感性。关键词肺炎支原体,快速培养法,咽拭子聚合酶链反应,血清抗体,配对研究-3-目录前言....................................................................................5第一章文献综述...............................................................6§.................................................................6§.........................................................6§.................................................................6§.........................................................6§.........................................................7§....................................................7第二章材料和方法............................................................8§.........................................................................................8§..................................................................................8§..............................................8§..................................................................................9§..............................................................................9第三章结果.......................................................................10第四章讨论.......................................................................11-4-结论...................................................................................12参考文献.............................................................................12致谢.....................................................................