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不同糖浓度对体外培养小鼠成纤维细胞增殖、分化的影响.doc

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不同糖浓度对体外培养小鼠成纤维细胞增殖、分化的影响.doc

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不同糖浓度对体外培养小鼠成纤维细胞增殖、分化的影响.doc

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文档介绍:临床医学论文-不同糖浓度对体外培养小鼠成纤维细胞增殖、分化的影响
                    作者:李盛,石汉平,孙冠青,孟锐
【摘要】 
【关键词】  创伤愈合成纤维细胞血糖应激反应   
Abstract:  Objective  To find the relationship between glucose concentration and the proliferation of   The proliferation of mouse fibroblast was tested in different concentration of glucose at 12h,24h,48h K amount of the outcell type I collagen fibers was tested by the mouse type I collagen Elisa kit,and the expression of αsmooth muscle actin (αSMA) tested by   We found that under the medium of low concentration,the proliferation was much more than that in the high concentration groups after 12h,24h and 48h(P<),namely the concentration was negatively correlated with the tested the outcell type I collagen fibers using the mouse type I collagen Elisa kit,but there was no difference between each group(P>).About the expression of mouse αSMA,positive correlation was found between the glucose concentration and the expression of the mouse α  At the original augmentation of the blood glucose,the proliferation of fibroblast is not when it gets over a level,the proliferation will be depressed,which can lead to the delay of the healing of raw surface;the concentration of glucose has nothing to do with the outcell type I collagen fibers;the concentration of glucose is a fact