文档介绍：2022 50 4 598-604
山西农业科学 ， （ ）： Journal of Shanxi Agriustica, were selected as objects
studied, by AGAR plate diffusion method and minimum inhibitory concentration(MIC), bacteriostasis of 8 pathogenic bacteria
and 1 fungus including Escherichia coli, Staphylococcus aureus, Staphylococcus albus, Bacillus subtilis, Bacillus thuringiensis,
Salmonella typhi, Streptococcus agalactiae, Salmonella and Fusarium oxysporum was detected and the differences of
bacteriostatic activity between the two kinds of BV were compared. A microplate reader and a fluorescence microscope were used
to verify the inhibitory effect of BV on Streptococcus agalactiae. The results showed the BV of A. cerana cerana and A. mellifera
ligustica had inhibitory effect on Gram-positive bacteria, fungi and some Gram-negative bacteria. As the concentration of BV
increased, the diameter of the bacteriostatic zone increased, and the bacteriostatic effect became more obvious. The minimum
inhibitory concentrations（MIC）of BV of A. cerana cerana and A. mellifera ligustica against Gram-positive bacteria and fungi
were both 5 mg/mL. The detecting data of the microplate reader showed that the OD value of the negative control group reached
after 12 h of culture, while the OD values of the BV with concentrations of , , mg/mL were only