文档介绍：Position of Forensic STR Markers
on Human Chromosomes
TPOX 13 CODIS Core STR Loci
D3S1358
TH01
D8S1179
D5S818 VWA
D2S1338 FGA D7S820
CSF1PO
AMEL
Sex-typing
Penta E
D13S317 D19S433
D16S539 D18S51 D21S11 AMEL
Penta D
经过筛选的STR基因
座,扩增条件基本相同,
可以在同一个PCR体系
中扩增多个靶基因座,
叫作复合扩增。
复合扩增可提高单次
检测的信息量,提高个
人识别率,降低成本和
检材的消耗,对微量检
材的鉴定特别有价值。
法***庭科学技术鉴定研究所
Fada Institute of Forensic Medicine & Science FDFS
STR检测试剂盒
法***庭科学技术鉴定研究所
Fada Institute of Forensic Medicine & Science FDFS
AB I 公司DNA分析仪大家族
•毛细管电泳式:310 、3100 、3700
ABI P RISM 310 ABI P RISM 3100 ABI P RISM 3700
遗传分析仪遗传分析仪遗传分析仪
ABI P RISM 377
测序仪 ABI PRISM
377 DNA Sequencer
ABI Prism 310 ic Analyzer
capillary
Syringe with
polymer solution
Injection
electrode -
Outlet Autosampler
buffer tray
+ Inlet
buffer
Human Identity Testing with Multiplex STRs
AmpFlSTR ® SGM Plus™ kit
Smaller
Homozygous at THO1 DNA Size (base pairs)
fragments Heterozygous at D16
D3 (1 peak)
amelogenin D8 TH01 (2 peaks) Larger
D19 VWA D21 D16 fragments
probability of a random D18 D2
match: ~1 in 3 trillion FGA
Results obtained in less than 5 hours with a
spot of blood the size of a pinhead
amelogenin D3 Male (2 peaks)
D19 Female (1 peak)
D8
Two different individuals individuals different different Two Two VWA D16
D21 D18 D2
FGA
TH01
Simultaneous Analysis of 10 STRs and Gender ID
法***庭科学技术鉴定研究所
Fada Institute of Forensic Medicine & Science FDFS
Human Identity Testing with Multiplex STRs
AmpFlSTR ® SGM Plus™ kit
Smaller
Homozygous at DNA Size (base pairs)
fragments Heterozygous at
D3 THO1 (1 peak)
amelogenin D8 TH01 D16 (2 peaks)Larger
D19 VWA D21 D16 fragments
probability of a random D18 D2
match: ~1 in 3 trillion FGA
Results obtained in less than 5 hours with a
spot of blood the size of a pinhead
amelogenin D3 Male (2 peaks)
D19 Female (1 peak)
D8 VWA D16
D21 D18 D2
FGA
TH01
Two different individual