文档介绍：第三军医大学硕士学位论文
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英文缩写英文全称中文全称
α-SMA Alpha-smooth muscle actin a-平滑肌肌动蛋白
Ab Antibody 抗体
BZ benzamidine hydrochloride hydrate 苯甲脒
plementary DNA 互补 DNA
Col I Collagen I I 型胶原
DAB Diaminobenzidine tetrahydrochloride 四盐酸二氨联苯***
DEPC Diethyl pyrocarbonate 焦碳酸二乙酯
DMEM/F12 o’s modified Eagle’s medium/F12(1:1) DMEM/F12 培养液
DMSO Dimethyl sulfoxide 二甲亚砜
dNTP deoxyribonucleoside triphosphate 脱氧核糖核苷三磷酸
EB Ethidium bromide 溴化乙锭
ECM Extracellular matrix 细胞外基质
Ep tube Eppendorf tube 微量离心管
FBS Fetal bovine serum 胎牛血清
HRP Horseradish peroxidase 辣根过氧化物酶
ICAM-1 intercellular adhesion molecule-1 细胞间粘附分子
IL-8 Interleukin-8 白细胞介素-8
MCP1 monocyte chemoattractant protein-1 单核细胞趋化蛋白-1
MFB myofibroblast 肌成纤维细胞
mRNA Messenger ribonucleic acid 信使核糖核酸
MTT Methylthio tetrazole 噻唑蓝
OD Optical density 光密度值
PAR2 Protease-activated receptor 2 蛋白水解酶激活受体-2
PBS Phosphate buffered saline 磷酸盐缓冲液
RNA Ribonuclunc acid 核糖核酸
RT-PCR Reverse transcription-polymerase chain reaction 逆转录-聚合酶链式反应
SABC Strept avidin-plex 链霉亲合素-生物素复合物
TGFβ Transforming growth factor β转化生长因子β
wt weight 重量
1
第三军医大学硕士学位论文
Effect of mast cell tryptase on cultured human renal fibroblasts
Abstract
Objective: Mast cell tryptase has been implicated in regulation of skin fibroblast, lung
fibroblast and hepatic stellate cell proliferation and collagen synthesis. In this study, we
observed the effect of mast cell tryptase on cultured human renal interstitial fibroblasts, so
as to further investigate the effect of tryptase on renal fibrosis.
Methods: Normal human renal interstitial fibroblasts were isolated from
nephrectomized microscopically normal renal tissue of malignant kidney tumor and
cultured in vitro. The cultured fibroblasts were identified by cell shape,
immunocytochemistry and electron microscope, and divided into following groups.
1. different concentration tryptase with equal heparin(wt/wt):
(1) 0ng/ml tryptase with 0ng/ml heparin
(2) 10ng/ml tryp