现在用的螺旋藻培养配方.xls

文档介绍：螺旋藻培养基
分子量老薛李修岭
成分浓度,mg/L g/L 100倍液(g/ 500 mL) 浓度,g/L 100倍液(g/ 500 mL) 用时取(mL/L)
Na2CO3 4030
NaHCO3 13610 840 10 mL
K2HPO4 500 25 25
NaNO3 2500 125 125
K2SO4 1000 1 50 1 50
NaCl 1000 1 50 1 50
MgSO4·7H2O 200 10 10
CaCl2·2H2O 40 2 2 10 mL
Vitamin B12
FeCl3·6H2O 10 mL
Na2-EDTA 75 4
FeSO4·7H2O
A5 solution 1 ml 1 mL 1 mL 1 mL
PIV PIV metal solution 6 ml
B6 solution 1 mL 1 mL 1 mL
Distilled water 990 ml
pH 不调pH 9
附:
A5 solution: mg/100mL g/L g/500 mL g/L g/100mL
H3BO3 286
MnCl2·4H2O 181
ZnSO4·7H2O 22
CuSO4·5H2O
NaMoO4·2H2O
Co(NO3)2·6H2O
Distilled water 100 ml 100 mL
PIV metal solution: mg/100mL
MnCl2·4H2O
ZnCl2
CoCl·6H2O
Na2MoO4·2H2O
Distilled water 100 ml 100 ml
B6 solution mg/L g/100mL
NH4NO3 230
K2Cr2(SO4)4 ·24H2O 96
NiSO4· 7H2O
Na2WO4· 2H2O
Ti2(SO4)3 40
Distilled water L
螺旋藻培养基
分子量
成分
Na2CO3
NaHCO3
K2HPO4
NaNO3
K2SO4
NaCl
MgSO4·7H2O
CaCl2·2H2O