文档介绍：程序名称:
木质纤维素生物质的酶法糖化
实验室分析程序
This procedure describes the enzymatic harification of cellulose from native or pretreated lignocellulosic biomass to glucose in order to determine the maximum extent of digestibility possible. A saturating level of mercially available or in-house produced cellulase preparation and hydrolysis times up to one week are used.
一、简介:
本实验描述,天然纤维素酶糖化或预处理木质纤维素生
物质为葡萄糖,以确定可能的最大消化率,一个在商业内部最高水平制备及水解纤维素酶所使用时间最长不超过一周。
一、范围:
本过程适用于木质纤维生物质。如果生物质中可能含一些淀粉,以干重量百分比去计算纤维素总葡聚糖时必须纠正为以淀粉含量减去总干重量葡萄糖的百分比;
所有分析的进行要按照一个适当的、具体的实验室质量保证计划(二次分配)。
二、术语:
生物质的预处理:生物质被化学或热量改变结构组成;
纤维素酶:一种酶的制备显示有三个纤维素阶段:远藤- 1 ,4 -β-的D -葡聚糖酶,外切- 1 ,4 -β-葡萄糖苷酶和β-的D -葡萄糖苷酶,它们在不同纤维素制备中出现活动程度不同。
三、意义和使用:
1 The maximum extent of digestibility is used in conjunction with other assays to determine the appropriate enzyme loading for the harification of biomass
2 This procedure can also be used to measure the efficacy of a given pretreatment based on a maximum enzyme loading
1 最大程度的消化率是用于与其他实验结合,以确定生物质糖化适当的酶量;
2 此过程也可以用来衡量一个最大的酶量为基础的特定预处理效果。;
四、干扰:
1 Test specimens not suitable for analysis by this procedure include acid- and alkaline-pretreated biomass samples that have not been washed. Unwashed pretreated biomass samples containing free acid or alkali may change solution pH to values outside the range of enzymatic activity; and the unwashed glucose in the biomass may influence the