紫外分光光度法测定橙汁中维生素C 的含量[J].pdf.pdf

文档介绍：安徽农业科学,Journal of Anhui Agri. Sci. 2011,39� 1�� 236 - 237,240 责任编辑张杨林责任校对卢瑶
紫外分光光度法测定橙汁中维生素 C 的含量
陈玉锋,庄志萍�, �
牡丹江师范学院化学化工学院黑龙江牡丹江 157012
摘要[目的]为市售饮料中维生素 C� Vc�的科学测定提供参考。[方法]利用 Cu2 + 催化溶解氧氧化 Vc,建立紫外分光光度法测定橙汁
中还原型 Vc 的方法,并对测定条件进行优化。[结果]采用磷酸和醋酸混酸溶液� 3∶ 7,V∶ V�作稳定剂,Cu2 + 为催化剂,70 ℃恒温水浴中,
测定市售橙汁饮品中 Vc 的含量,标准曲线方程为 y = 0. 071 4x + 0. 135 0,线性相关系数为 0. 999 5。该方法精密度 RSD� n = 5�为
0. 35% ,加标回收率为 98. 5%。[结论]建立了紫外分光光度法测定橙汁中 Vc 的方法,该方法简单、快速,结果令人满意。
关键词维生素 C�紫外分光光度法�橙汁
中图分类号 S123 文献标识码 A 文章编号 0517 - 6611� 2011� 01 - 00236 - 02
Determination of Vitamin C in Orange Juice by UV Spectrophotometric Method
CHEN Yu-feng et al � Chemistry Department,Mudanjiang Normal University,Mudanjiang,Heilongjiang 157012�
Abstract [Objective]The aim was to provide references for the scientific determination of Vitamin C � Vc� in sold beverage. [Method]
With Cu2 + as catalyst to oxidize Vc,the method to determine reduced Vc in orange juice by UV spectrophotometry was constructed,and then
the determination conditions were optimized. [Result]With the mixed solution of phosphoric acid and acetic acid � 3∶ 7,V∶ V� as the stabiliz-
er,Cu2 + as catalyst and in the 70 ℃ water-circulator bath,Vc content in the sold orange juice was determined,the standard curve equation
was y = 0. 071 4x + 0. 135 0,the linear correlation coefficient was 0. 999 5,the precision RSD� n = 5� was 0. 35% and recove