文档介绍：munications
Biosynthesis of anofluorine molecule
A fluorinase enzyme has been discovered that catalyses carbon–fluorine bond formation.
lthough fluorine in the form of fluo- uitious of the small class anofluorine acetate by 19F-NMR analysis and HPLC.
ride minerals is the most pounds and has been identified in The fluorination reaction seems to involve
Ahalogen in the Earth’s crust, only 12 more than 40 plant species from all of the a nucleophilic attack by fluoride ion at the
naturally - continents apart from Antarctica4, but its C-5፱ carbon of SAM, generating 5፱-FDA
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pounds have so far been found , and how biosynthetic fluorination pathway has not and itantly displacing L-methion-
these are biosynthesized remains a mystery2. been clearly defined2,5. Fluoroacetate is also ine. The mechanism by which 5፱-FDA is
Here we describe an enzymatic reaction produced by the bacterium S. cattleya6 metabolized to fluoroacetate remains to
that occurs in the bacterium Streptomyces when it is grown in culture medium sup- be established (Fig. 1), although fluoro-
cattleya and which catalyses the conversion plemented with fluoride ions. acetaldehyde7,8 may be the immediate pre-
of fluoride ion and S-adenosylmethionine We investigated this process of enzymatic cursor of fluoroacetate.
(SAM) to 5፱-fluoro-5፱-deoxyfluoroadeno- fluorination by incubating a partially puri- David O’