文档介绍：条斑紫菜(Pyropia yezoensis)10个选育系的ISSR分析
摘要:利用issr分子标记进行条斑紫菜(pyropia yezoensis)10个选育品系的种质鉴定研究。结果表明,具条带特异性且表达稳定清晰的issr引物共14个,%,这些引物可用作区分这些品系的分子标记,同时利用引物826扩增的特异性位点建立了这些选育品系的issr遗传识别码。
关键词:条斑紫菜;内部简单序列重复;种质鉴定
中图分类号:q945 文献标识码:a doi编码:.1006—
issr analysis of ten breeding lines of pyropia yezoensis
chen shu—yin, lu qin—qin, zhang mei—ru, hu chuan—ming, xu guang—ping
(marine fisheries research institute of jiangsu province, nantong, jiangsu 226007,china)
abstract: inter simple sequence repeat(issr)—pcr was used to analyze the ic variation and germplasm identification of ten different breeding lines of pyropia yezoensis. 14 polymorphic and specifical primers were screened and optimized. from the generated issr products by these primers, % of the dna bands amplified were polymorphic. these primers could be used as molecular ic markers to discriminate and evaluate the ten lines. a number of peculiarity amplified bands of primer 826 were applied to establish the issr code patterns of the p. yezoensis ten lines.
key words: pyropia yezoensis;issr;germplasm identification
条斑紫菜(pyropia yezoensis)为我国长江以北海区主要栽培的大型经济海藻,在经济藻类产业中占有重要地位。随着栽培规模的不断扩大及海域环境变化,条斑紫菜生产面临着产量不稳、产品质量参差不齐及病害频发等的挑战。保护优良种质资源,开发具抗逆性好及生长速度快等性状的新品种、新品系,是当前条斑紫菜产业可持续发展的重要研究课题。为有效实施紫菜种质资源的保护及科学开发利用,国家级紫菜种质库保存有以条斑紫菜为主的大量紫菜原始种质及育种材料,并对优良的野生或栽培品系进行选择、纯化与扩增培育,为紫菜优良种质的研究与有效利用提供丰富的物质基础。