文档介绍：山东人学硕士学位论文
中文摘要
日本扁柏Chamaecyparis obtusa( .)、韩国半岛等地,在我国大陆地区只有零星分布,大多为人工种植,作为景观植物。该植物含有丰富的挥发性成分,近年来同韩等地兴起森林浴,其意图就是利用该植物的挥发性成分治疗一些皮肤病等。但是生态环境对日本扁柏品质、挥发油含量及其化学成分的影响较大,为探讨采自韩国同本扁柏的挥发油成分和生物活性,以及主要化学成分,本论文研究了采自韩国的同本扁柏(Chamaecyp obtusa)鲜叶的挥发油成分及次代谢产物的化学成分。
首先,本试验对日本扁柏鲜叶挥发油化学成分进行分析,,从而揭示挥发油化学成分,为日本扁柏推广和应用提供科学依据。气相色谱条件:(30 , pm);载气为高纯氦气, 柱流量60mL/rain;分流比:l:80;进样口温度230。C,程序升温,起始温度60。C, 保持l rain,以5。C/min的升温速率升至200。C,保持15min。质谱条件:EI离子源, 离子源温度230。C,四极杆温度150。C,接口温度280℃;质量扫描范围30"-'550 alTlU; 进样量:1“L。共鉴定出43种化合物,主要为酯类、烯醇类及烯烃类化合物,还有少量烯酮、烷烃、烷酸、烯醛类等化合物。各种烯烃类化合物为挥发油中的主要活性物质,是生物活性较强或芳香气味的主要组成成分,在日用化工、医药、食品、香精香料工业中具有重要价值和应用意义。同时,对提取的挥发油进行了初步体外抗菌活性筛选。
其次,本试验对采自韩国的同本扁柏叶化学成分进行了研究。日本扁柏叶甲醇提取浸膏,通过萃取,得到极性不同的石油醚相、氯仿相和正丁醇相。对氯仿相进行分离,采用各种柱层析方法得到7种化合物。通过质谱和核磁共振等方法进行鉴定,确定其化学结构。为进一步确定日本扁柏的应用提供物质基础依据。
关键词: 日本扁柏;挥发油;抗菌;化学成分
ABSTRACT
Chamaecyparis obtusa( .)Endl.(is extensively distributed in me mountainous area of Japan,Korea Peninsula and Taiwan Province of t11is plant
was cultivated in main land of China for leaf is a rich source of essential
recent years,the essential oil isolated from the leaves of
Chamaecyp D6如心口was
used to cure the fungal infection of skin in the aims of
screening natural
resources for antibacteral and anitfungal agents,the chemical constituents of the essential oil from the leaves of Chamaecyp obtusa were investiganted by the
phytochemical constituents were also investiganted. In addition,the cheIIlical and
biological activity researchs of the Chamaecyparis species were also reviewed.
The chemical constituents and bioactivities of the Chamaecyp Dbt”sP were investigated in this volatile oil of Chamaecyp obtusP丘om Korean is extracted by normal pressure water-steam distillation and analyzed by gaS chromatography-mass spectrometry method,and pounds have been
ponents include lipids, enols, alkenes and so OIL F