文档介绍：NF-κB抑制剂PDTC对小鼠急性心肌梗塞后Wnt信号通路的影响
作者:黄莺1,马依彤1,杨毅宁1,刘芬2,陈邦党2,李晓梅1 作者单位:国家自然科学基金项目(30760263);新疆维吾尔自治区重点实验室项目(XJDX0903) 1 新疆医科大学第一附属医院冠心病科,新疆乌鲁木齐 830054;2 新疆心血管病研究所
【摘要】目的:探讨核转录因子(NF)-κB抑制剂二硫代氨基甲酸吡咯烷(PDTC)对小鼠急性心肌梗塞(AMI)后Wnt信号通路的影响。方法:结扎3月龄雄性C57/BL小鼠左冠状动脉建立AMI模型,随机分成AMI+PDTC组(36只,术后每日腹腔注射PDTC 120mg/kg),AMI组(36只,每日注射同上述剂量的生理盐水)。所有小鼠按心梗时间分3 d、7 d、14 d亚组,比较应用PDTC后NF-κB信号通道相关的p65,Wnt信号通路的Dishellved(dvl)-1 mRNA表达,糖原合成激酶(GSK)-3β总水平及磷酸化水平变化,β-连接蛋白(catenin)及缝隙通道连接蛋白(connexin)43表达。结果:与AMI 组相比,AMI+PDTC组的p65,dvl-1 mRNA表达显著降低(P均<),p/t GSK-3β 3 d,7 d及14 d时均显著升高();βcatenin表达3 d时显著低于AMI组,7 d时显著高于AMI组();connexin 43表达显著降低(P=)。结论:抑制NF-κB活性、信号通道后Wnt信号通路相关蛋白活性也发生变化,说明急性心肌梗塞后NF-κB信号通路与Wnt信号通路之间存在互通作用。
【关键词】心肌梗塞;NF-κB;小鼠
Effects of NF-κB inhibitor-PDTC on Wnt signal pathway in mice postinfarction /HUANG Ying,MA Yitong, YANG Yining,LIU Fen,CHEN Bangdang, LI Xiaomei//Department of Coronary Artery Disease, The 1st Affiliated Hospital,Xinjiang Medical University,Urumqi,Xinjiang,830054,China
Corresponding author: MA Yi-tong, Email:myt-xj@
Abstract:Objective:To study the effects of NF-κB inhibitor -PDTC on expression of protein related to Wnt signal pathway in mice post-:Choose 3 months C57/BL mice to set up acute myocardial infarction animal model by ligation of left coronary artery .They were divided to AMI+PDTC group(36 cases, mice was treated with injected PDTC on dose of 120 mg/kg·d)and AMI group (36 cases, mice was treated with injected physiological saline with same volume).They were divided into 3 subgroups according to time postinfarction: 3d, 7d, 14d post infarction and detected expression of p65 related to NF-κB signal pathway by immunohistochemistry; and protein related to Wnt signal pathway: dvl-1 mRNA by RT-PCR, total (t)and phosphorylation (p) level of glucogen synthesis kinase(GSK)-3β,β-catenin,connexin 43 by Westernblot in left ventricular. Results: Expression of p65 decreased in AMI+PDTC group (P<). Compared with