文档介绍：现代食品科技 Modern Food Science and Technology 2012, ,

桑叶中 1-脱氧野尻霉素分离纯化的工艺研究

罗蕾蕾,吴俊
(湖北武汉军事经济学院军需系给养勤务教研室,湖北武汉 430035)
摘要:本文以 1-脱氧野尻霉素(DNJ)的含量和提取率为考察指标,对使用 AB-8 大孔树脂和 732H 型阳离子树脂分离纯化桑叶
中 DNJ 的工艺进行了研究。使用 AB-8 大孔树脂对提取液进行脱色除杂处理,上样液 pH 值为 ,上样量不超过 2 BV,流速为 1 BV/h,
可将提取液中 DNJ 含量从 %提高到 %,回收率为 98%。采用 732H 型阳离子树脂层析法进一步纯化,分段洗脱流程为:1 BV
去离子水- M 氨水-2 M 氨水,洗脱流速为 2 BV/h,当流出液 pH 为 9~10 时,DNJ 被洗出。产品中 DNJ 含量为 %,
此工艺纯化效果好,方法简单,能够为工业化生产高浓度 DNJ 提取物提供基础工艺数据。
关键词:桑叶;1-脱氧野尻霉素;大孔树脂;阳离子树脂;纯化
文章篇号:1673-9078(2012)12-167-169
Isolation and purification of 1-deoxynojirimycin from Morus alba L. leaves
LUO Lei-lei, WU Jun
(Quartermaster Department of Military Economics Academy, Wuhan430035, China)
Abstract: This paper studied the technology for isolation and purification of 1-Deoxynojirimycin (DNJ) from Morus alba L. leaves by
using AB-8 macroporous resin and 732 H type cation exchange resin sequentially, with the content and recovery rate of DNJ as indexes. The
result showed that AB-8 resin was suitable for the preliminary isolation and purification of DNJ. At pH , the maximum loaded amount of
Morus alba L. leaves extract was 2BV and the recovery rate of DNJ was 98% by collecting the solution flowing out at 1BV/h as flowing rate.
The content of DNJ was enhanced to %. 732H type cation exchange resin chromatography was used to