文档介绍：液相色谱-串联质谱法快速测定粮谷中的矮壮素和敌草快残留量
曹慧收稿日期: 修订日期:
基金项目:浙江省科技厅分析测试科技计划项目(No 2011C37072);浙江省质量技术监督系统科研计划项目(No 20110203)
作者简介:曹慧(1980- ),女,浙江湖州人,工程师,主要从事色谱和质谱检测与科研工作。E-mail:@。
,陈小珍,张东雷,祝颖,武晓光
( 浙江省质量检测科学研究院食品检测部,杭州 310013 )
摘要:采用液相色谱-串联质谱技术(UPLC/MS/MS)快速分析粮谷中的矮壮素和敌草快残留量。样品经甲醇-水-乙酸溶液提取,HLB固相萃取小柱净化,用液相色谱-串联质谱法测定,外标法定量。结果表明,矮壮素和敌草快在5~50μg/L范围内线性关系良好;%~%之间,%~%之间;两者相对标准偏差均小于15%;/kg,定量下限分别为10μg/kg和20μg/kg。该方法完全满足粮谷中矮壮素和敌草快残留量的快速分析。
关键词:液相色谱-串联质谱;敌草快;矮壮素;粮谷
中图分类号: 文献标识码:A 文章编号:
Determination of the Residues of Chlormequat and Diquat in Grains Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
CAO Hui, CHEN Xiao-zhen, ZHANG Dong-lei, ZHU Ying, WU Xiao-guang,
( Zhejiang Institute of Quality Inspection Science, Hangzhou 310013, China )
Abstract: A method was developed for determination of the residues of chlormequat and diquat in grains using ultra performance liquid chromatography-tandem mass spectrometry (UPLC/MS/MS). The samples were extracted with methanol-water-acetic acid solution, passed through HLB cartridge to clear-up and determined by UPLC/MS/MS using external standard method. The linear range was 5μg/L ~50μg/L and the correlation coefficient (r2) was more than . The average recoveries were in the range from % to % for chlormequat. The average recoveries ranged from 73