文档介绍：现代食品科技
Modern Food Science and Technology
2011, ,
蚕蛹蛋白酶解制备抗氧化肽的初步研究
李高扬,崔堂兵,陈亮
(华南理工大学生物科学与工程学院,广东广州510006)
摘要:将蚕蛹通过粉碎和丙酮脱脂后,得到蚕蛹蛋白粉。用碱性蛋白酶、中性蛋白酶、胃蛋白酶、木瓜蛋白酶、胰蛋白酶酶解蚕蛹
蛋白粉,以DPPH清除能力为指标对酶解过程进行分析,结果表明胃蛋白酶对蚕蛹蛋白有较好的酶解效果。然后通过正交优化胃蛋白酶的
酶解条件,并研究水解产物多肽的体外抗氧化活性。
关键词:蚕蛹蛋白;酶解;抗氧化;肽
文章篇号:1673-9078(2011)7-810-814
Preliminary Study of Enzymatic Hydrolysis of Silkworm Pupa Protein for
Production of Antioxidant peptide
LI Gao-yang, CUI Tang-bing, CHEN Liang
(School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 51006, China)
Abstract: Silkworm pupa was crushed and defatted by acetone, giving silkworm pupa powder. Then the powder was hydrolyzed with five
proteases (Alkali protease, neutral protease, pepsin, papain, trypsin). Among the tested enzymes, pepsin was confirmed as an optimal enzyme
preparation to prepare silkworm pupa polypeptide in terms of DPPH· scavenging activity of hydrolysates. The enzymatic hydrolysis procedure was
optimized using orthogonal array method and the corresponding product was subjected to further analysis of antioxidant activity, such as reducing
power and O2·, ·OH and DPPH·scavenging activities.
Key words: silkworm pupa protein; enzyme hydrolysis; antioxidation; peptide
生物活性肽(bioactive peptides)是指来源于细菌,真
菌,植物,动物等,对生物机体的生命活动有益或具有
生理作用的肽类化合物,是蛋白质中天然氨基酸以不同
组成和排列方式构成的从二肽到复杂的线性、环形结构
的不同肽类的总称[1]。自