文档介绍：实用口腔医学杂志(J
Pract
Stomat01)2012 May,28(3)
・279・
不同诱导环境对牙周膜干细胞膜片生物学特性的影响
刘一涵赵喜聪张勇杰刘洪臣
朱斌
【摘要】
目的:探讨不同诱导体系对牙周膜干细胞(periodontal ligament
stem
cells,PDLSCs)膜片的构建以及生物学性能的影
响。方法:分离培养大鼠发育期根端复合体(developing
apical
complex,DAC)细胞,隔天更换收集细胞培养液卜清并过滤冻
存(DAC条件培养液)。分离培养人PDLSCs,并扩增构建PDLSCs细胞膜片,利用DAC条件培养液和成骨诱导液分别诱导
PDLSCs膜片5、IO、15 d。通过倒置显微镜、HE染色、免疫组化、RT—PCR检测经过诱导的人PDLsCs细胞膜片形态学和功能
学变化。结果:与成骨诱导相比,DAC诱导后膜片含有丰富短棒状细胞和大量细胞外基质(extracellular matrix,ECM),细胞膜
片ALP和periastin表达丰富,体外成骨基因表达更高。结论:与成骨诱导体系相比,DAC诱导体系更适合PDLSCs膜片的形成。
【关键词】
牙周膜干细胞;细胞膜片;发育期根端复合体;诱导
Effects of different induction systems
periodontal ligament stem cell sheets
Ⅱ￡,Yihanl,ZHAO Xicon92,ZHANG
ro埘ic2,UU
Hongchenl,ZHU Bin‘.
Belting。Department of
Stomatology,Chinese
P“General
Hospital,China;
of
Stomatology,The Fourth Military Medical Uni-
venity,Xian
【Abstract】Objective:To
study the effects of different induction systems
the construction and ion of periodontal ligament
stem cell
:Developing plex(DAC)of
14 day・aged SD
separated gently and in vitro cultured with
d—MEM and 10% medium
changed every
days and
supernatant of the used medium
prepared for
DAC conditioned ligament stem
cells(PDLSCs)were
obtained and identification,PD/LsCs were
subcuhured,induced by
DAC conditioned medium and osteogeneefic induction respectively;PDISC sheets
sheets
examined by inverted microscope,HE staining,RT-PCR,and
:PDLSC sheets formed in
different induction systems sheet under DAC induction showed more extracellular
matrix(ECM)and
cells,expressed
higher livel of and osteogenic genes than that under osteogenic :DAC imtuction
and biofunctiun of PDLSC sheet.
-2the formation
【Key words】Periodontal ligament
-28stem cells;Cell sheet;Developing plex;Induction
may
prom